Flow Cytometry and Cell Sorting Facility

Michael Gregory – Facility Director
Ellen Rothenberg – Faculty Advisor

The Caltech Flow Cytometry and Cell Sorting Facility is a multi-user facility which provides expert assistance and advanced instrumentation to researchers for analyzing and separating various types of cells and micro-organisms according to their measurable properties of light scatter and fluorescence. The mission of the Flow Cytometry and Cell Sorting Facility is to provide flow cytometry resources to solve complex biological problems and promote cutting edge research on a fee-for-service basis. The facility strives to provide cost effective analysis and cell separation on several different platforms using a myriad of protocols to provide a competitive advantage for Caltech researchers and to enhance the scope and quality of scientific research performed at Caltech.

The facility is currently equipped with four cell sorters: a Sony SY3200, a BD FACS Aria Fusion, a Beckman Coulter Cytoflex SRT, and a Union Biometrica BioSorter. The facility also houses two flow cytometers: a Miltenyi Biotec MACSQuant VYB, and a Beckman Coulter Cytoflex S.

Here are the basic steps to request service:

· Fill out our Flow Cytometry Project Form .

· We will then schedule a consultation and determine the proper instrumentation based on your experiment.

· After consultation, you will then be able to schedule your time directly with an operator for facility-run experiments or schedule your training on user-run instruments.

· Reach out to [email protected] with any questions!

All publications that include results or products generated in the Caltech Flow Cytometry & Cell Sorting Facility must include the following acknowledgement:

"Data used in this (manuscript, presentation, or other format) was generated in the Caltech Flow Cytometry & Cell Sorting Facility. Our Research Resource Identifier number is RRID: SCR_025087."

For additional comprehensive information about the Facility, click the box below.

Michael Gregory

Facility Director

• B167 Church
• Mail Code 156-29
• (626) 395-8046
• [email protected]

Maddy Adolf

Cytometry Technician

• B165 Church
• (626) 395-3998
• [email protected]