Programmable Molecular Technology Center

Prof. Niles Pierce – Principal Investigator
Dr. Natalie Kegulian – Molecular Technologies Specialist
Cody Newman – NUPACK Software Engineer

The Molecular Technologies resource applies principles from the new field of dynamic nucleic acid nanotechnology to develop and support programmable molecular technologies for imaging the molecules of life (DNA, RNA, proteins, and complexes thereof). Signal amplification based on the mechanism of hybridization chain reaction (HCR) provides a unified framework for 10-plex, quantitative, high-resolution imaging of RNA, protein, and protein:protein complex targets in highly autofluorescent samples. HCR imaging offers a unique combination of multiplexing, quantitation, sensitivity, resolution, penetration, versatility, and robustness.

Molecular Technologies resource development focuses on enabling multiplex, quantitative, high-resolution imaging of the interactome and advancing the performance and versatility of the HCR imaging platform.

MT Support: [email protected]

The NUPACK resource is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving researchers in the emerging disciplines of molecular programming, nucleic acid nanotechnology, synthetic biology, and across the life sciences. NUPACK algorithms have pioneered the treatment of complex and test tube ensembles containing arbitrary numbers of interacting strand species, providing crucial tools for capturing concentration effects essential to analyzing and designing the intermolecular interactions that are a hallmark of these fields. The all-new NUPACK cloud web app facilitates rapid job submission and result inspection with scientific algorithms running in parallel in the scalable NUPACK hybrid cloud.

NUPACK resource development focuses on developing physically sound, mathematically rigorous, computationally efficient analysis and design tools that enable increasingly reliable and sophisticated molecular engineering.

NUPACK support: [email protected]