The Biological Imaging Facility (BIF, formerly called the Biological Imaging Center, BIC) is the only core facility on the Caltech campus providing light microscopy resources. We are located in the basement (room B133) of the Caltech Beckman Institute which, together with the Arnold and Mabel Beckman Foundation, support the BIF. The BIF has 10 microscopes. Seven are confocal microscopes for optical sectioning of fluorescently labeled samples. Five are Zeiss laser scanning confocal microscopes (LSMs), one is a Leica STELLARIS 8 FALCON confocal system with TauSense and the last is a Nikon Spinning Disc confocal. The Leica FALCON module allows the system to do fluorescence lifetime imaging microscopy (FLIM) and 5 HyD detectors, combined with a white light laser (WLL), can image fluorophore emissions out to 850 nm. The LSM 880s have the Fast Airyscan module for higher speed and/or super-resolution microscopy as well as providing a higher signal to noise ratio. The LSM 980 has the next generation Fast Airyscan 2 and Definite Focus 2. Two LSMs and the Leica STELLARIS 8 DIVE have multiphoton/two-photon lasers (MP, a category of nonlinear optics, NLO) which allow for deeper imaging of tissues. Two have non-descanned detectors (NDDs) for greater sensitivity. The Nikon microscope is also set up for doing Total Internal Reflection Fluorescence (TIRF) microscopy and the super-resolution technique using Stochastic Optical Reconstruction Microscopy (STORM). The seventh microscope is a Leica DMI 6000 wide field fluorescence microscope for deconvolution analyses. The last two microscopes are Light Sheet Fluorescence Microscopes (LSFM, also called selective/single plane illumination microscope, SPIM) that provide high speed optical sectioning. One is a LaVision Biotec Ultramicroscope II, the other is a Zeiss Z.1. Seven microscopes are equipped with incubation chambers for controlling temperature and/or CO2 levels. For more information click on the link below to the Biological Imaging Facility website.